J01749 product description one of the most commonly used cloning vectors, pbr322 confers resistance to ampicillin and tetracycline. The cole1type plasmid pbr322 is commonly used as vector tool in molecular biology. Thermo scientific pbr322 is one of the most commonly used li cloning vectors. As a starting material for cloning of casein gene sequences. The dna sequence of the entire plasmid has been published. This is a free resource for the scientific community that is compiled by addgene. S1 protectionsi mapping was performed by the procedure of weaver and weissmann 15. Definicion, historia, propiedades, caracteristicas. The plasmid pbr322 was one of the first ek2 multipurpose cloning vectors to be designed and constructed ten years ago for the efficient cloning and selection of recombinant dna molecules in. Plasmids pbr322 and puc8 pbr322 plasmid one of the first plasmids to be used in recombinant genetics was called pbr322. Plasmid pbr322 was one of the first multipurpose cloning vectors constructed for use in e. Plasmid vector pbr322 and its specialpurpose derivatives. Plasmid pbr322 confers resistance to ampicillin and tetracycline.
The molecule is a doublestranded circle 4,361 base pairs in length 2. The rop gene product, which regulates plasmid replication by stabilizing the interaction between rnai and rnaii transcripts, maintains the copy number at about 20 per cell. Created in 1977 in the laboratory of herbert boyer at the university of california, san francisco, it was named after francisco bolivar zapata, the postdoctoral researcher who constructed it. Rna synthesised in uitro, or total rna isolated from bacteria containing pbr322, was ethanolprecipitated. Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. The p stands for plasmid, and br for bolivar and rodriguez. This plasmid is derived from the cole1type plasmid pmb1 and shares the same type of replication mechanism and controls as cole1 and relatives.
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